Lactobacillus gasseri DNA lab quality control

Texnik xususiyatlari

Qadoq	Frozen vial; 10 ^ 7~10 ^ 8copies/mL;10 tubes/box
Ozuqa muhiti	Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃)
Qo‘llash tartibi	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
O‘stirish sharoiti	37 ℃;24-48h;5% CO2
Saqlash sharoiti	2-8 ℃
Xavfsizlik darajasi	1
Isolation	human intestine
Qo‘llanilishi	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated bacterial suspension of Lactobacillus galactoris, and can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. through digital PCR value.