Klebsiella pneumoniae DNA lab quality control

Texnik xususiyatlari

Qadoq	Frozen vial; 10 ^ 7~10 ^ 8copies/mL;10 tubes/box
Shakli	The colony has a diameter of 1-2mm, round, neat edges, opaque, gray-white front, smooth surface, raised in the middle, bright surface, moist texture, easy to pick up, G-(red), bacilli, purity: pure
Ozuqa muhiti	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Qo‘llash tartibi	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction.
O‘stirish sharoiti	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic atmosphere;
Saqlash sharoiti	-80 ℃
Xavfsizlik darajasi	2
Morphology	The colony has a diameter of 1-2mm, round, neat edges, opaque, gray-white front, smooth surface, raised in the middle, bright surface, moist texture, easy to pick up, G-(red), bacilli, purity: pure
Qo‘llanilishi	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of Klebsiella pneumoniae, which can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. by adding stabilizer and digital PCR fixed value.