Streptococcus pneumoniae DNA lab quality control

Texnik xususiyatlari

Qadoq	Frozen storage tube; 10 7~10 8copies/mL;20 tubes/box
Ozuqa muhiti	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Qo‘llash tartibi	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μl.
O‘stirish sharoiti	37 ℃,18-24h,5% CO2 + 95% air
Saqlash sharoiti	-80 ℃
Xavfsizlik darajasi	2
Isolation	sputum of 75-year-old male, Phoenix, AZ
Qo‘llanilishi	The liquid indoor quality control is Streptococcus pneumoniae inactivated bacteria suspension, through fluorescence quantitative pcr combined with digital PCR fixed value, can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.